Home | About us | Editorial board | Ahead of print | Current issue | Archives | Search | Submit article | Instructions | Subscribe | Advertise | Contact us |  Login 
National Journal of Maxillofacial Surgery
Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 541

Table of Contents
Year : 2021  |  Volume : 12  |  Issue : 3  |  Page : 361-366  

Effect of platelet-rich fibrin versus chitosan-based Axiostat hemostatic agent following dental extraction in cardiac patients on antiplatelet therapy: A comparative study

1 Department of Dentistry, ESICMC PGIMSR Medical College, Rajajinagar, Bengaluru, Karnataka, India
2 College of Dentistry, Jazan University, Kingdom of Saudi Arabia
3 Department of Oral and Maxillofacial Surgery, Drs. Sudha and Nageswara Rao Siddhartha Institute of Dental Sciences, Vijayawada, Andhra Pradesh, India
4 Department of Pharmacology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India
5 Department of Dentistry, BKL Walawalkar Rural Medical College and Hospital, Chiplun, Maharastra, India

Date of Submission07-Dec-2020
Date of Decision24-Dec-2020
Date of Acceptance16-Apr-2021
Date of Web Publication13-Dec-2021

Correspondence Address:
Dr. Vidushi Sharma
Assistant Professor, Department of Pharmacology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana - 133 207, Ambala, Haryana
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/njms.NJMS_270_20

Rights and Permissions

Background: Platelet-rich fibrin (PRF) is a biomaterial that promotes wound healing. It has a fibrinous matrix wherein platelets, pro-inflammatory cytokines, and various growth factors along with few cells are entrapped while Chitosan is a naturally occurring cationic biopolymeric material that is derived from an animal product, chitin. It has demonstrated biological properties which include acceleration in wound healing, hemostasis, enhancement of immunological response, mucosal adhesion by eliciting biological responses, and anti-microbial action.
Aim: The aim of this study was to evaluate the effect of PRF and Axiostat (A chitosan-based product) on hemostasis after tooth extraction among cardiac patients on antiplatelet medication.
Materials and Methods: This prospective study was carried out on 300 patients undergoing tooth extraction. Participants were divided into two categories (n = 150, respectively) as Group I (PRF dressing) and Group II (Axiostat dressing). Time to achieve hemostasis was observed using a stopwatch. Average pain score calculation was performed using visual analog on the 7-day postoperative period. Descriptive statistics were done, and data analysis was performed using the Mann–Whitney U-test. P < 0.5 and < 0.001 were considered statistically significant and extremely significant, respectively.
Results: Average pain score was 1.86 ± 0.06 in Group I and 1.05 ± 0.87 in Group II. Thus, lower postoperative pain was seen with Axiostat dressing. Hemostasis was achieved in Group II participants in 1.25 ± 0.06 min and in 1.89 ± 0.54 min in Group I. P < 0.01 was obtained, although no statistically significant difference in postoperative pain scores (P = 0.8) was seen.
Conclusion: Chitosan is a superior wound dressing material in achieving hemostasis in cardiac patients on antiplatelet medication after tooth extraction.

Keywords: Axiostat, cardiac, extraction, hemostasis, pain, platelet-rich fibrin

How to cite this article:
Rajendra K, Vempalli S, Kadiyala M, Sharma V, Karipineni S, Gunturu S, Patil DB. Effect of platelet-rich fibrin versus chitosan-based Axiostat hemostatic agent following dental extraction in cardiac patients on antiplatelet therapy: A comparative study. Natl J Maxillofac Surg 2021;12:361-6

How to cite this URL:
Rajendra K, Vempalli S, Kadiyala M, Sharma V, Karipineni S, Gunturu S, Patil DB. Effect of platelet-rich fibrin versus chitosan-based Axiostat hemostatic agent following dental extraction in cardiac patients on antiplatelet therapy: A comparative study. Natl J Maxillofac Surg [serial online] 2021 [cited 2022 Dec 4];12:361-6. Available from: https://www.njms.in/text.asp?2021/12/3/361/332317

   Introduction Top

Platelets have an enucleated spherical or oval-shaped structure that contains granule. They have a diameter measured between 200 and 500 nm. These α-granules contain transforming growth factor-β (TGF-β), insulin-like growth factor-I, and platelet-derived growth factor (PDGF). The secretion of active proteinaceous components allows granular fusion with the cellular membrane of the platelets by their subsequent binding to transmembranous cell membranous receptors.[1]

Tooth extractions performed in patients with cardiac surgeries requiring placement of artificially made heart valves are placed under anti-coagulant therapy. These patients are at an increased risk of complications arising because of hemorrhage occurring postoperatively. Most commonly used anti-coagulant drugs include warfarin and acetylsalicylic acid.[2]

Medications targeted against platelets are mainly aspirin (at a dosage of 75 mg to 300 mg), dipyridamole, and Clopidogrel. The drug combination of aspirin along with Clopidogrel results in an additive and synergistic effect due to their inhibitory effect on complementary pathways of the platelet pathway.[3]

The international normalized ratio (INR) is commonly used to assess anti-coagulant control with a maximum value of 3.5 (range between 3 and 4). Stopping of anti-coagulation therapy and subsequent, replacement with heparin before a minor surgical procedure can result in thromboembolism. Various protocols have been proposed which include (a) combination therapy using anti-fibrinolytics and hemostatic agents, (b) no changes in the regular anti-coagulant therapy or by administering tranexamic acid as local anti-fibrinolytic agent, and (c) use of fibrin glue.[2] Local hemostatic agents have been classified into (a) passive, (b) active, (c) fluids, and (d) sealants. Passive agents have been found to trigger the clotting cascade through absorption and aggregation while the active agents are found to contain clot components while it contains clot components while the remaining two classes include poly-ethylene glycol polymer, fibrin-containing adhesive, glutaraldehyde, cyanoacrylate, and albumin. They are also been classified on the basis of their mechanisms of action into (a) factors responsible for coagulating plasma, (b) fibrinolytic inhibitors, (c) agents causing vasoconstriction and which are pro-aggregation, (d) agents responsible for the stimulation of cellular aggregation along with adhesiveness, (e) agents responsible for causing denaturation of proteins, and (f) mixed pathways.[4] There have been numerous hemostatic agents that are used for hemostasis, for example, collagen, oxidized regenerated cellulose, platelet-rich fibrin (PRF), etc.[5]

The American College of Chest physicians have suggested that patients undergoing anti-coagulant therapy must discontinue the use of drug 5 days before any surgical intervention and bridge therapy utilizing the use of heparin should be initiated. However, in contrast, the American Heart Association has suggested that the INR should be reduced within a range of 2–2.5 with strict monitoring of INR. Harfoush et al. evaluated 50 patients with an INR value of ≤3.5. Of these, 25 patients constituted Group A in which the extraction sockets were packed with PRF, whereas Group B (n = 25) was treated with dry gauze packing. Eighty-percent of patients belonging to Group A had a moderate amount of bleeding seen in 28% cases, whereas 72% suffered from severe bleeding. Hence, it was concluded from the study that topical PRF application following the extraction of teeth in participants on warfarin therapy helped in achieving adequate hemostasis following the extraction of teeth.[6]

The use of techniques that make the use of platelet concentrates is a simple yet optimal method.[1] A first-generation platelet concentrate, platelet-rich plasma (PRP) was introduced by Marx in 1998. The disadvantage of PRP is that (a) there is no uniformity in the preparation technique, (b) it requires activation using bovine-origin thrombin, and (c) anti-coagulants have to be added.[7],[8] On the other hand, PRF is prepared by immediate centrifuging of venous blood. The serum obtained is termed as “hyper-acute serum” and has a high proliferative effect on bone marrow-derived mesenchymal stem cells, chondrocytes, and osteoblasts. However, despite the obvious regeneration causing effects, there are certain drawbacks which are as follows: (a) the PRF handling should be done in an open and sterile environment. This is most of the times impossible to achieve in an oral environment and (b) it does not have adequate tensile strength, hence, the material cannot be sutured.[8]

Although due to legal restrictions, a second-generation concentrate of platelets was developed by Choukroun et al. in France in 2001 which is known as “PRF.” This biomaterial has no requirement of an anti-coagulant or a bovine origin thrombin. It is an autologous fibrinous matrix that contains the large quantities of cytokines derived from the platelets. It has been routinely used for the healing of hard along with soft tissues.[2]

Autologous bone graft materials containing high concentrations of platelets such as the PRP and PRF. The PRP is an autologous platelet concentration which has been suspended within plasma and contains a variety of growth factors such as the PDGF, TGF-β1 and-β2, vascular endothelial growth factor (VEGF) while the PRF is a next-generation concentrated platelet product comprised leukocytes, glycoproteins, cytokines, and a fibrinous matrix.[9] PRF influences three mechanisms that are involved in the process of wound healing: (i) angiogenesis (ii) immunological response, and (iii) proliferation of epithelium. It causes the release of elevated levels of three growth factors: PDGF-AB, VEGF, IL-1 β, and TNF-α (last two are pro-inflammatory cytokines).[10] PRF is a bioactive wound dressing and has an advantage in being of autogenous nature.[11] The main factor responsible for obtaining a good quality PRF membrane is time elapsed between blood collection and centrifugation during which polymerization takes place due to the thrombin component. A network of fibrin is formed due to the transformation of soluble form of fibrin to insoluble fibrinous form. However, a significant disadvantage is the rapid degradation of the PRF membrane in a short time period when compared to the time taken for the healing of the bone.[12]

Chitosan is a biopolymeric mucopolysaccharide with a structural resemblance to glycosamine which is an alkaline deacetylated chitin product. It is derived from crustacean exoskeletons.[13] Biochemically, chitosan comprises of a high-molecular-weight polymer along with glucosamine and acetyl glucosamine when it is subjected to solubility in diluted acids (organic as well as inorganic). Chitosan has a number of biological properties due to its biocompatible, biodegradable, anti-bacterial, and easy degradation to glucosamine components.[3] The biodegradable properties of chitosan result in the production of nontoxic oligosaccharides of variable lengths which can be processed in the different pathways of metabolism and can be excreted from the body. Structurally, chitosan is a nontoxic, bio-degradable complex carbohydrate chitin-derivative poly β (1-4)-N-acetyl D-glucosamine. It has lesser than 100% deacetylated content, which makes it a lesser co-polymer that contains both D-glucosamine and N-acetyl-D-glucosamine monomeric units. It exhibits mucoadhesive properties in its acidic salt form.[14]

Preparations derived from chitosan have varying amount of molecular weights. Its properties include antimicrobial activities, hemostasis, healing, acting as a scaffold for tissue engineering and as systems for the delivery of drugs. One such commercially available product is HemCon bandage which is used for controlling bleeding of wounds. Chitosan is a positively charged material resembling sponge and bears a hemostatic property. Its mechanism of activity invokes attracting red blood cells and platelets (which have surface negative charge). Thus, using ionic interactions, chitosan forms a strong seal at the site of a surgical wound.[2] Malette et al. demonstrated that a solution of chitosan led to the formation of coagulum when it comes in contact with blood.[15] The anti-microbial effects of chitosan may be explained under the following mechanisms: (a) its binding with DNA through the protonated amino groups and (b) interaction with the negatively charged bacterial cell membrane.[16]

The aim of the study was to compare the efficacies of PRF with chitosan-based Axiostat on controlling hemostasis in the extraction sockets in cardiac patients undergoing antiplatelet therapy.

   Materials and Methods Top

This was a prospective study conducted on 300 participants who were indicated for the extraction of teeth. All the study participants had a medical history of cardiac disease and were on anti-platelet drug therapy. The inclusion criteria of the study were as follows: (1) Patients with an age range of 35–70 years, (2) patients with a INR value between 1 and 3.5, i.e., ≤3.5, and (3) patients were nondiabetic. The exclusion criteria were as follows: (1) Patients who had to undergo more than one tooth extraction and (2) patient with known allergy to sea-foods.

The study participants were randomly divided into two groups containing equal numbers of participants, i.e., n = 150, each in Group I (PRF) and Group II (Axiostat), respectively. All patients underwent extractions after preventive antibiotic prophylaxis before extractions. For prophylactic measures, Amoxicillin in dose of 2 g by oral route was given prior to the procedure. A single operator performed the extraction procedure along with the placement of the selected hemostatic agent within the socket.

In Group I, PRF membrane was placed after preparing it in clean and sterile glass test tubes using centrifugation at 3000 rpm for 10 min. In Group II, commercially available Axiostat dressing was placed within an extraction site followed by the application of digital pressure for a duration of 30 s. After hemostasis was evident, the material was removed followed by irrigation with saline. Time to achieve hemostasis was noted using a stopwatch. All patients were provided with postextraction instructions and were asked to report in case of any postsurgical or operative bleeding was observed. Postextraction healing of soft-tissue changes was observed on the 7th postoperative day. The average pain score was calculated using a Visual Analog Scale from 1 to 10 after seven days of postoperative period.

Descriptive analysis was done after entering all the collected data in Microsoft Excel sheets and the statistical tool used for analyzing data was Mann–Whitney U-test. P < 0.5 and 0.001 were set as significant and extremely significant, respectively.

Ethical approval

This study was conducted in compliance with the protocol; ethical approval was obtained from the Institutional Ethical Committee (IEC/IC26/2020).

   Results Top

The average pain score was found to be 1.86 ± 0.06 in Group I when compared to 1.05 ± 0.87 in Group II; therefore, with slightly lower postoperative pain in Axiostat treated group [Graph 1]. Complete hemostasis was achieved in Group II participants (extraction sockets were packed with Axiostat) in 1.25 ± 0.06 when compared to Group I (PRF-treated extraction site) with a mean ± standard deviation value of 1.89 ± 0.54 [Graph 2]. A statistically significant value of P < 0.01 was obtained on comparing both the groups. However, in this study, no statistically significant difference in postoperative pain (P = 0.8) was observed [Table 1].

Table 1: Parameters studied and their P values

Click here to view

   Discussion Top

In the current study, complete hemostasis was seen to be achieved earlier in chitosan-based wound dressing compared to the extraction socket site which was packed with PRF and a statistically significant P value (<0.01) was obtained on comparison of both groups. Although no statistically significant difference in the postoperative pain was observed, the study findings are corroborated by a study conducted by Sarkar et al. who compared the efficacy of PRF and Chitosan hydrogel in 60 patients who were on oral anti-platelet medication therapy. The time in which hemostasis was achieved was noted. It was seen that in extraction sites which were treated with platelet enriched fibrin, bleeding stopped in 2.64 min while in the group treated with Axiostat, arrest of bleeding was seen in 1.182 min. There was a statistically significant difference in the time of stoppage of bleeding between both the comparison groups studied (P < 0.0001). The postoperative pain was found to be lesser in sites treated with PRF when compared to Axiostat hydrogel.[17]

In the present study, Chitosan was found to be a superior wound dressing material in achieving hemostasis in cardiac patients on antiplatelet medication after tooth extraction and these results were in accordance with the study conducted by Seethamsetty et al. who evaluated that surgical sites treated with Chitosan-based dressings had better hemostatic effects along with lower pain as compared to the control group.[18] Supportive findings have been reported by Malmquist; however, their findings did not show any statistical significance. An interesting observation made was that if an extraction socket was fully packed, there was an increase in pain due to residual unreactive acetic acid.[19]

Larik et al. compared the effectiveness of PRF with Transexamic acid in 84 patients. These materials were tested after placement within extraction sockets. 97.6% efficacy in patients (41//42) of Group A was seen, whereas 92.86% patients in Group B demonstrated clinical effectiveness. No statistical significance with a P value of 0.306 was seen.[20]

Bhujbal et al. in a split-mouth study which was performed on 20 study participants who underwent bilateral mandibular third molar extractions compared PRF treated sites with normal controls. Healing of soft tissues was evaluated immediately, first, third, and 7 days of the postoperative period, whereas bone density was measured using radiographic assessment. On the 3rd- and 6th-month postoperative follow-ups, postoperative swelling was found to be statistically significant in the immediate period, 1st, 3rd, and 7th days (P < 0.15, < 0.58 and < 0.78, respectively) but no significance was obtained on day 7 (P < 0.00). The postoperative healing of the soft and hard tissues was seen in the PRF site.[6]

Sinha et al. evaluated the efficacy of Axiostat on 50 patients with cardiac medical history disease without any alteration or stoppage of anti-platelet drug therapy. The average time taken hemostasis was 1.5 min in cases with prolonged bleeding.[3] The self-adhesive property of Axiostat is mainly because of electrostatically driven binding of the red blood cells to the chitosan component of the Axiostat dressing by the formation of a densely packed viscous material which by itself acts as a pressure pack within an extraction socket. The poly-cationic property of this chitosan-based dressing material at an acidic pH helps it in causing disruption of cell membranes of Gram-negative bacteria.[3]

Sharma et al. examined a total of forty participants who were on anti-platelet medication. The split-mouth study design was applied with the sites of extractions categorized into: (a) Group 1: those participants receiving Axiostat dental dressing (study group) and (b) Group 2: Participants with the conventional method of wound dressing using a sterile gauze pack with biting force and suturing was done, if it was indicated. It was observed that sites of extraction which were treated with Axiostat attained hemostasis in the lesser time period (mean period = 1 min 13 s) when compared to the control (mean = 14 min 1 s). A statistically significant difference (P < 0.0001) was observed between the time of hemostasis and postoperative pain along with better healing (P < 0.0001).[21]

Kale et al. evaluated the efficacy of a HemCon dental dressing for control of bleeding from pos-extraction sockets. It was found that extraction sockets treated with HemCon achieved hemostatic control in a mean period of 53 s when compared with controls with a mean period of 918 s. This was having a statistical significance of a P value of 0.001. Roughly, 72.5% of extraction sites treated with this dressing demonstrated better healing in the postoperative period with a statistical significance of a P < 0.001. In addition, it was observed that this material showed better results in sites with active bleeding.[22]

Eldinbury in his study on 20 patients undergoing tooth extraction compared PRF dressing (Group A) with HemCom Dressing (Group B). Complete achievement of hemostasis was found in both the groups and no delay in bleeding was observed. Participants belonging to Group A demonstrated very less pain and an accelerated rate of healing while those in Group B exhibited moderate-to-severe pain in the first few days after the extraction procedure along with retardation of the healing process.[2] Similar findings were also reported by Fradet et al. who observed that grafts treated with chitosan were impermeable to blood flow.[23]

De Almeida Barros Mourão et al. in their study reported a mean time for achieving hemostasis as 10.3 ± 2.5 s.[24] An interesting observation was made by a comparative study between collocate (a collagen dressing) and PRF by Sharma et al. They concluded that both of these materials cause an acceleration in the wound healing process and a significant reduction in pain as well.[25]

   Conclusion Top

In this study, Axiostat has been demonstrated to have effective value in lowering bleeding time among postextraction cases in patients on anti-platelet therapy. Furthermore, a reduction in the postoperative pain and comorbid conditions were seen which has been supported by various investigators. Hence, it can be concluded that chitosan-derived wound dressings have better results in the healing of extraction sockets.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

   References Top

Khiste SV, Tari RN. Platelet-rich fibrin as a biofule for tissue regeneration. ISRN Biomat 2013:1-6. [doi: 10.5402/2013/627367].  Back to cited text no. 1
Eldibany RM. Platelet rich fibrin versus Hemcon dental dressing following dental extraction in patients under anticoagulant therapy. Tanta dent J 2014;11:75-84.  Back to cited text no. 2
Sinha N, Mazumdar A, Mitra J, Sinha G, Baunthiyal S, baunthiyal S. Chitosan based axiostat dental dressing following extraction in cardiac patients under antiplatelet therapy. Int J Oral Health Med Res 2017;3:65-7.  Back to cited text no. 3
Budko EV, Chernikova DA, yampolsky LM, Yatsyuk VY. Local hemostatic agents and ways of their improvement. Biol Herald 2019;27:274-85.  Back to cited text no. 4
Belkhede SG, Salaria SK, Aggarwal R. Comparative evaluation of the platelet-rich fibrin bandage versus gelatin sponge-assisted palatal wound healing of free gingival graft donor site: A case series. J Indian Soc Periodontol 2019;23:589-92.  Back to cited text no. 5
[PUBMED]  [Full text]  
Harfoush M, Boutros E, Al-Nashan A. Evaluation of topical application of platelet rich fibrin (PRF) in homeostasis of the bleeding after teeth extraction in patients taking warfarin. Int dent J St Res 2016;4:144-7.  Back to cited text no. 6
Naik B, Karunakar P, Jayadev M, Marshal VR. Role of platelet rich fibrin in wound healing: A critical review. J Cons Dent 2013;16:284-94.  Back to cited text no. 7
Kardos D, Hornyák I, Simon M, Hinsenkamp A, Marschall B, Várdai R, et al. Biological and mechanical properties of platelet-rich fibrin membranes after thermal manipulation and preparation in a single-syringe closed system. Int J Mol Sci 2018;19:3433-47.  Back to cited text no. 8
Bhujbal R, Veerabhadrappa SK, Yadav S, Chappi M, Patel V. Evaluation of platelet-rich fibrin and platelet-rich plasma in impacted mandibular third molar extraction socket healing and bone regeneration: A split-mouth comparative study. Eur J Gen Dent 2020;9:96-102.  Back to cited text no. 9
  [Full text]  
Jain V, Triveni MG, Kumar AB, Mehta DS. Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft. Contemp Clin Dent 2012;3:S2401-1.  Back to cited text no. 10
Gür ÖE, Ensari N, Yılmaz NDS. The effects of platelet-rich fibrin membrane on secondary healing after pleomorphic adenoma excision in the hard palate: A preliminary study. Ann Maxillofac Surg 2017;7:78-81.  Back to cited text no. 11
Aktas A, Ozer T, Sen M, Turk M, Karabulut E. Comparison of the mechanical properties of platelet-rich fibrin and ankaferd blood stopper-loaded platelet-rich fibrin. Niger J Clin Pract 2018;21:1087-92.  Back to cited text no. 12
[PUBMED]  [Full text]  
Rao SB, Sharma CP. Use of chitosan as a biomaterial: Studies on its safety and hemostatic potential. J Biomed Mater Res 1997;34:21-8.  Back to cited text no. 13
Pusateri AE, McCarthy SJ, Gregory KW, harris RA, cardenas L, McManus AT, et al. effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of svere venous hemorrhage and hepatic injury in swine. J Trauma 2003;54:177-82.  Back to cited text no. 14
Malette WG, Quigley H, Gaines RD, Johnson ND, Raines G. “Chitosan”: A new hemostatic. Ann Thoracic Surg 1983;36:55-8.  Back to cited text no. 15
Maksym PV. Chitosan as a hemostatic agent: Current state. Eur J Med 2015:24-33. [doi: 10.13187/ejem.s.b 2015.2.24].  Back to cited text no. 16
Sarkar S, Prashanth NT, Shobha ES, Rangan V, Nikhila G. Efficacy of Platelet Rich Fibrin versus chitosan as a hemostatic agent following dental extraction in patients on antiplatelet therapy. J Oral Biol Craniofac Res 2019;9:336-9.  Back to cited text no. 17
Seethamsetty S, Sarepally G, Sanober A, Qureshi Y, Fatima U, Arif SM. A comparative evaluation of the effectiveness of chitosan-based dressing and conventional method of hemostasis in patients on oral antithrombotic therapy without therapy interruption. J Pharm Bioallied Sci 2019;11:S18-23.  Back to cited text no. 18
Malmquist JP, Clemens SC, Olen HJ, Wilson SL. Hemostasis of oral surgery wounds with the HemCon dental dressings. J Oral Maxillofac Surg 2008;66:1177-83.  Back to cited text no. 19
Larik MD, Hassan SG, Majeedano SA, Rajper WA, Waq AS, Akro B, et al. Hemostatic effect of platelet rich fibrin versus Tranexamic acid after tooth extraction in patients under anticoagulant therapy. Open Access J Biomed Sci 2020;2:404-7. [doi: 10.38125/OAJBS.00187].  Back to cited text no. 20
Sharma S, Kale TP, Balihallimath LJ, Motimath A. Evaluating effectiveness of axiostat hemostatic material in achieving hemostasis and healing of extraction wounds in patients on oral antiplatelet drugs. J Contemp Dent Pract 2017;18:802-6.  Back to cited text no. 21
Kale TP, Singh AK, Kotrashetti SM, Kapoor A. Effectiveness of HemCon dental dressing versus conventional method of hemostasis in 40 patients on oral antiplatelet drugs. Suktan Qaboos Univ Med J 2012;12:330-5.  Back to cited text no. 22
Fradet G, Brister S, Mulder DS, Lough J, Averbach BL. Evaluation of chitosan as a new hemostatic agent: In vitro and in vivo experimants. Chitin Nat Technol 1986;4:443-5.  Back to cited text no. 23
de Almeida Barros Mourão CF, Calasans-Maia MD, de Mello Machado RC, de Brito Resende RF, Alves GG. The use of platelet-rich fibrin as a hemostatic material in oral soft tissues. Oral Maxillofac Surg 2018;22:329-33.  Back to cited text no. 24
Sharma V, Kumar A, Puri K, Bansal M, Khatri M. Application of platelet-rich fibrin membrane and collagen dressing as palatal bandage for wound healing: A randomized clinical control trial. Indian J Dent Res 2019;30:881-8.  Back to cited text no. 25
[PUBMED]  [Full text]  


  [Table 1]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Article Tables

 Article Access Statistics
    PDF Downloaded190    
    Comments [Add]    

Recommend this journal